Contract Laboratory has received the following Biotechnology Laboratory Test Requests which need to be fulfilled! These Laboratory Test Requests are received from a variety of sources ranging from start-up companies to large, multinational Fortune 500 corporations, international organizations, well-respected universities and government agencies that are actively seeking Independent, Third-Party Biotechnology Laboratories to perform their Biotechnology Testing, Analysis, Assays, Product Development, Scientific Research Experiments, Inspections, Certifications, and Engineering Projects. If you are a Biotechnology laboratory that would like more information on these Biotechnology projects, please visit our registration page or call toll us free 1-855-377-6821.
Biotechnology laboratory needed for the following:
Construction and screening of partially random (15, site specific randomization in 45 nucleotide long sequences) oligonucleotide expression libraries for specific protein-protein interactions.
1., Oligonucleotide (~ 40-50 bases long) syntheses, and random mutagenesis at every third (defied) positions.
2., Oligonucleotide sequencing.
3., Inserting oligonucleotides into vectors and expressing into oligopeptides.
4., Expression library screening for specific protein-protein interactions (phage display or bacterial two-hybrid methods)
5., Cloning, monoclonal protein production
6., Characterization of peptide interactions (Kd, specificity)
Lab Opportunity Type:
lab licensure, contract R&D,
Lab Opportunity Description:
We are looking for a number of modern biotech laboratories (and companies, world-wide) for further development and marketing a Novel Method to Design and Produce Specifically Interacting Proteins. The procedure is simple, un-expensive, clone-based and high throughput.
Biro J: System and method to obtain oligo-peptides with specific high affinity to query proteins. US Patent Application Publication US 2007/0015189 A1, January 18, 2007.
Jan C Biro MD. PhD
Professor, Homulus Fnd
Preclinical laboratory needed for bioanalytical blood coagulation assays (including PT, APTT, TT and FIB) in the presence of a potential therapeutic compound. Of interest would be whether this compound promotes, inhibits(in a dose dependent manner) or does not affect the blood coagulation cascade. We will would provide the experimental design/parameters.
Biotechnology / biopharmaceuticals company needs Preclinical and toxicology studies rG-CSF
1) in vitro receptor binding assays (comparative to reference product)
2) in vivo rodent models (neutropenic and non-neutropenic), compare test and reference product
28-day repeat dose tox with local tolerance
(repeated dose tox, toxicokinetics, and local tolerance.)
1) in vitro comparative bioassays (e.g. receptor-binding studies, cell proliferation assays)
2) in vivo: erythrogenic effects of test product and the reference product should be quantitatively compared in an appropriate animal assay (polycythaemic and/or normocythaemic mouse assay)
3-month repeat dose tox with local tolerance in appropriate species (e.g. rat or dog)
Virology and immunology laboratory needed for Viral re-activation in mice. Infect mice with polyomavirus. Allow virus infection to become latent (~6 mo). Treat mice with immunosuppressive test drugs and assay for virus re-activation.
Polyoma virus is BSL2. virus titers from blood, organs.
In vitro testing of our lead compound against HIV-1:1) A fusion assay according to Cavrois et al 2002 (Nature biotechnology 20.1151-1154)2) A CD4 ELISA assay, according to Herrera et al 2003 (Journal of Virology 77.1084-1091)3) An assay to assess anti-HIV activity of our lead compound, co-transfecting cells with vesicular stomatitis virus (VSV) as a control to confirm specifity of our compound with respect to HIV4) As assay to assess inhibition of radio-labelled chemokine ligands to CD4/CXCR4 and CCR5
GMP Compliant lab to perform the following: Full Monograph Testing (individual and total) for the following items:·,Magnesium Stearate·,Maltodextin·,Polyethylene Glycol·,Silicon Dioxide,·Stearic Acid·,Croscarmellose Sodium,,Dibasic Calcium Phosphate·,Lactose Monohydrate·, Specific Rotation for Ephedrine HCl·,Diphenhydramine HCl Organic Nitrogenous Bases for a pharmaceutical company
assess the androgenic activity, in a quantitative fashion, of a single steriod with the trivial name tetrahydrogestrinone(THG) in a pilot scale study. Either an aquatic organism model or rodent model would appear adequate to provide the information desired but other potential testing types will be considered such as in-vitro receptor binding studies. The study should compare the relative androgenicity of THG to a reference compound such as testosterone
Bioanalytical Laboratory needed for Vitamin detection on Fetal Bovine Serum. Following is a list of vitamins and detectable units:
Vitamin B-12 pg/mL
Folic Acid ng/mL
Vitamin B-6 ng/mL
Thiamin B-1 ng/mL
Vitamin H Biotin pg/mL
Riboflavin B2 ng/mL
Vitamin A Retinol ng/mL
Vitamin C ng/mL
Microbiology Laboratory needed for microbial analysis SB 3112. The test material consisted of only the bacterial spores at a count of 55 x 107 cfu/ml. The tests were run in compliance with the following protocols: 1 USEPA FIFRA;GLP Standards 40 CFR 160 2 USEPA TSCA 40 CFR 792 3 Org. Economic Coop. and Dev. Principles of GLP, Annex 2, C(97)186 4 Japan Ministry of Ag, Forestry & Fisheries, Notification No. 59 Noshan 3850, Director-General of Agricultural Prod. Bureau, 10 Aug 84
Preclinical toxicology laboratory needed for Cytotoxicity studies: MEM Elution Sample Amount: 120 cm2 or 4 grams Irritation: Intracutaneous Reactivity (ISO) (w/2 extracts) Sample Amount: 240 cm2 or 8 grams Sensitization: Magnusson-Kligman (w/2 extracts) Sample Amount 600 cm2 or 32 grams (6 samples minimum) Sensitization: Local Lymph Node Assay: Sample Amount 400 cm2 or 20 grams