Contract Laboratory has received the following Biotechnology Laboratory Test Requests which need to be fulfilled! These Laboratory Test Requests are received from a variety of sources ranging from start-up companies to large, multinational Fortune 500 corporations, international organizations, well-respected universities and government agencies that are actively seeking Independent, Third-Party Biotechnology Laboratories to perform their Biotechnology Testing, Analysis, Assays, Product Development, Scientific Research Experiments, Inspections, Certifications, and Engineering Projects. If you are a Biotechnology laboratory that would like more information on these Biotechnology projects, please visit our registration page or call toll us free 1-855-377-6821.
Test Request 17-01696
Bioanalytical Laboratory needed grow Human Primary Trophoblast cells with serum and without serum for varying amounts of time (0, 24hrs, 48hrs, 72hrs, and 96hrs). Then immunofluorescence stain the cells with GLUT8 and Humanin for images.
We are looking to have a data point for every combination of serum, time,and staining:
serum: with and without
time(hrs): 0 (control), 24hrs, 48hrs, 72hrs, and 96hrs
staining: GLUT8 and Humanin (if possible, also co-staininng with lysosomal markers)
We also need to have a non-isotype IgG negative control.
For immunofluorescence images, we would like at least 40x magnification.
Test Request 17-01577
Agriculture biotechnology company needs EPA GLP environmental toxicology laboratory for 4 ecotoxicology studies for a botanical active substance under EU requirements:
- Biodegradable test (OECD 301)
- Chronic toxicity study on bees
- Bee larvae brood study
- Typhlodromus piry, Aphidius rophalosiphi, Chrysoperla carnea, Coccinela sectempunctata.
We would really appreciate a quotation for these four tests.
Test Request 17-01518
Virology laboratory needed for performing PCR analysis for many different rabbit and porcine viruses based on the guideline (CPMP_BWP_3354_99), as follows. CPMP_BWP_3354_99: http://www.ema.europa.eu/docs/en_GB/document_library/Scientific_guideline/2009/09/WC500003222.pdf:
For rabbit viruses: Rabbit rotavirus, Reovirus type 3, Poxviruses, Rabbitpox (RPXV), Myxomatosis virus (MYXV), Shope fibroma virus, Rabbit haemorrhagic disease virus (RHDV), Rabbit papillomaviruses, Rabbit kidney vacuolating virus, Herpes cuniculi Adenovirus, Encephalomyocarditis virus, Borna disease virus, Sendai virus, Simian Parainfluenza (SV-5), Pneumonia virus of mouse (PVM).
For porcine viruses: Enveloped Viruses, Influenza virus A (,airborne), Nipah Virus & Menagnel Virus (paramyxoviruses), Vesicular Stomatitis Virus (aerosols), Pseudorabiesvirus (dsDNA, traumatic inoculation), Rabies virus (ssRNA, bite), West Nile virus(mosquito bite), Hantavirus (Rodent-borne), Eastern equine encephalomyelitis (mosquito bite) Non Enveloped Viruses, Swine Hepatitis E virus HEV(33 nm, RNA, fecal-oral), Encephalomyocarditis virus EMCV(28 nm RNA, oral), Swine Vesicular Disease Virus SVDV/PEV9 ( oral), Foot and mouth diseases virus FMDV (28nm, RNA, airborne), Reo/Rota virus (oral).
Life Science company needs laboratory for NMR spectras to help determine if there are steroimpurities. Spectras at NMR: 1H-13C HSQC 400-500MHz. We need one spectrum at room temperature and another at a different temperature (>15C degree difference). So 4 spectra total, 1H, 13C and at two temperatures.
Test Request 17-01212
LONG-TERM TESTING Bioscience company needs bioanalytical laboratory for raw material, in-process, and final product MAT or cell-based endotoxins testing with assays that are not based on the LAL or rProtein C methods for endotoxin detection. The liquid formulation of both proteins and polymers. We would need to have endotoxin testing performed with a MAT or cell-based assay, since we already know that protein interferes with the LAL and rProteinC methods.
Biosciences company needs bioanalytical laboratory for in-vitro anti-biofilm activity testing by Minimal Biofilm Eradication Concentration (MBEC) assays using peg lid containing biofilm plates on aqueous samples. Biofilm producing bacteria of interest are: initially - staph aureus, with others to follow.
Test Request 17-01071
LONG-TERM TESTING FDA GMP bioanalytical laboratory needed for developing and validating bioassays in bulk substance high-titer antibody biologics (PPM sensitivity) including bioanalysis for antifoam residuals and an
assay for Pluronic F68 excipient level (quantitative). Osmolality by vapor pressure.
Test Request 17-00840
Bioanalytical laboratory needed for 3rd party verification of a cryopreserved amniotic allograft tissue sample using an automoted cell counter: Countess II FL. A simple tri pane di exclusion test
The sample will be shipped next day on dry ice. We will need the testing to happen soon after arrival. The sample should be kept at -80 degrees celsius or in a liquid nitrogen freezer until testing is ready.
Once ready to test sample, simply pull the sample out and let thaw at room temperature.
Test Request 17-00729
University researcher needs pharmaceutical microbiology laboratory for cultured streptococcus mutans research study on the biochemical properties and antibacterial action of two drugs on the microbe culture from saliva samples including glucose utilization, DNA content (increased or decreased due to the effect of these drugs) and membrane integrity of strep mutans in the culture.
Test Request 17-00460
Test Request 17-00446
Molecular Biology / Bioanalytical Laboratory needed for up to three university-based experiments involving human cell lines:
Experiment #1: Test up to 5 human cell lines (i.e. liver, kidney, etc...). Formalinize these cell lines in order to have a bank ready to be used. Then culture peripheral blood stem cells,apply the different formalin treated cells into the growth media, and determine what occurs. I am looking to determine if differentiation is stimulated toward the cell lines that were added. Is there inhibition or is there no reaction?
Experiment #2: Culture peripheral stem cells, add various growth factors (ex. neupogen), and determine if cell expansion is stimulated
Experiment #3: Growth of autologous stem cells on an autologous feeder layer (cells and feeder layer from the same patient). Looking to find what numbers of cells are achieved in a set time frame.