USP Chondroitin Sulfate Sodium Testing
Project Description
Independent Third-Party Laboratory needed for USP Dietary Supplement Testing to the USP analysis of chondroitin sulfate sodium.
COMPOSITION CONTENT OF CHONDROITIN SULFATE SODIUM Standard solutions: 1.5, 1.0, and 0.5 mg/mL of USP Chondroitin Sulfate Sodium RS in water Sample solution: Transfer 100 mg of dried Chondroitin Sulfate Sodium to a 100-mL volumetric flask, dissolve in 30 mL of water, and dilute with water to volume. Diluent: Weigh about 297 mg of monobasic potassium phosphate, 492 mg of dibasic potassium phosphate, and 250 mg of polysorbate 80, and transfer to a 1-L beaker. Dissolve in 900 mL of water, and adjust with potassium hydroxide or phosphoric acid to a pH of 7.0 ± 0.2. Dilute with water to 1 L, and mix thoroughly. Titrimetric system (See Titrimetry <541>.) Mode: Photometric titration Titrant: 1 mg/mL of cetylpyridinium chloride in water. Degas before use. Endpoint detection: Turbidimetric with a photoelectric probe Analysis Samples: Standard solutions, Sample solution, and Diluent Transfer 5.0 mL each of the Standard solutions and the Sample solution to separate titration vessels, and add 25 mL of Diluent to each. Stir until a steady reading is obtained with a phototrode either at 420, 550, or 660 nm. Set the instrument to zero in absorbance mode. Titrate with Titrant using the phototrode to determine the endpoint turbidimetrically. From a linear regression equation, calculated using the volumes of Titrant consumed versus concentrations of the Standard solutions, determine the concentration of chondroitin sulfate sodium in the Sample solution. Calculate the percentage of chondroitin sulfate sodium in the portion of Chondroitin Sulfate Sodium taken: Result = (C/CU) × 100 C = concentration of chondroitin sulfate sodium in the aliquot of the Sample solution, obtained from the regression equation (mg/mL) CU = concentration of Chondroitin Sulfate Sodium in the Sample solution (mg/mL) Acceptance criteria: 90.0%-105.0% on the dried basis Thank you,
Project Information
Number:20-02104