Influenza and Corona Virus Environmental Treatment Study

Project Description

Large Air Handling and Treatment Company needs Environmental Microbiology Laboratory for conducting human influenza and corona family virus viral material testing of environmental swabs after treatments to the following Test Methods:
Below I report the method of detection of viral material at an environmental level taken from a scientific article and we need to do a test like this:

The surface samples were taken using nylon swabs, which were immersed in viral transport medium (VTM) before sampling. The standard sampling area size was 10?×?10 cm (swab applied in horizontal followed by vertical and diagonal sweeps).
Four air samples were taken during the study period, two samples at two different times of the day in week 5 and one sample in weeks 6 and 7. The air particles were collected using an Impactor FH5® sampler (Klotz GmbH, Germany) with filtration [24]. The sampler was positioned at approximately 2 m from the floor in the passenger security check area and ran for 33 min. The flow rate through the sampler was 30 L/min giving a total sample volume of 1000 L filtered through a gelatin filter paper (Gelatin Filter Disposables, Sartorius Stedim Biotech GmbH, Germany). Before nucleic acid extraction, an approximately 0.5?×?5 cm strip of the gelatin filter paper was immersed and dissolved in 1 ml VTM.
All samples were transported refrigerated and stored in refrigerator at approximately +?4 °C for short-term storage (maximum 24 h), and then frozen at approximately -?60 °C for extended storage before nucleic acid extraction and virus detection.