Laboratory Test Request

Project Description

Preclinical laboratory needed for performing a number of in vitro studies using stromal vascular fraction (SVF) cells.These studies involve labeling of SVF cells with magnetic microparticles and superparamagnetic iron oxide nanoparticles (SPION).

We want to measure the following parameters:

1-Assess cell viability, self -renewal capacity and proliferation capacity. of SVF cells exposed to SPION.
2-Confirm the intracellular uptake of SPION by SVF cells using one or more of the available techniques to determine intracellular iron or SPION content per cell (colorimetric assays: Prussian blue assay, Ferrozine assay; ICP-MS; ferromagnetic resonance; magnetophoresis; MACS).
3- Assess the ability of cells to retain the label throughout several cell divisions (dilution effect).

I will be happy to share any additional information with you in relation to the aforementioned techniques that will be required to perform this set of experiments.

I would appreciate your response ASAP including a quote and available earliest start date for these studies.

You can reach me at your earliest convenience to my cell phone (Cell:+1-630-362-5835), via email: pablogurman@gmail.com, or
Skype: pablogurman

Looking forward to hear from you soon.

Many thanks in advance.

Best regards,

Pablo Gurman, MD
Director
Gur &Gor LLC

Project Information

Number:16-01937

Industry:Biotechnology

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