Project Description

India Pharmaceutical Science Researcher needs Pharmacology Contract Research Organization for Pharmacology Study Experiments:

AIM AND OBJECTIVES OF THE SCIENTIFIC REASERCH WORK
The study has been designed to serve the following objectives:
1. To explore the critical role of selected antioxidants, minerals and vitamins on gastro protection by using various antiulcer screening models and biochemical parameters in rats.
2. To compare the effect of selected antioxidants, minerals and vitamins in GI disorders along with other reference gold standard drugs.
3. To recommend a safe therapy in managing gastric disorders based on preclinical studies.

METHODOLOGY OF THE REASERCH WORK
? Screening models:
1. Ethanol- Induced Ulcer Model in rats.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
? Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
2. Pylorus Ligation Induced Ulcer Model in rats.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
? Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
3. Cold Restraint stress Induced Ulcer (CRU) Model in rats.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
? Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
4. A small animal model of human Helicobacter pylori infection.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
5. Indomethacin induced ulcer model
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes


following 8 Groups of animals (n = 6) will be used in study