Project Description

FDA Microbiology Laboratory needed for cytotoxicity testing and antiviral testing for antiviral properties of one test product. The challenge virus will be Vericella zoster and the antiviral properties of the product will be assessed using a Post-Treatment test. The Post-Treatment test will determine if the product has inhibitory activity on the virus propagation in the cells. Prior to the antiviral testing, the cytotoxicity will be determined (TC50) to identify the highest non-cytotoxic concentration for this study. For the Post-Treatment test, the challenge virus dilutions will be incubated with the cells for 60 minutes, and the product concentrations will then be plated onto infected cells. The inhibitory concentrations of the chemicals (IC50 and/or IC90) will also be determined Best if using GraphPad Prism 5.0 or 6.0 software. Two replicates of cytotoxicity and antiviral testing will be performed.