Project Description

Biological biocide manufacturer needs microbioogy and genetics laboratory for biocide testing. In the production range we have three biological insectidies and one biological fungicide a basis of the bacteria Bacillus thuringiensis ( www.bioec.rs).
We need some laboratory analysis, so if your laboratory can do any of the following analysis please send us offer.

Necessary analysis
Identification of microorganisms of Bacillus thuringiensis:
1. Phenotypic methods
- physiological methods
- metabolic methods
2. Chemotaxonomic methods
- typing - serotyping on H14 antigen
- profile of all cellular proteins : PAGE, multilocus enzyme electrophoresis
3. Genotypic methods
- typing on DNA-based ( DNA fingerprinting), for example: AFLP-PCR
- typing ribosomal RNA

SDS-PAGE (gel electrophoresis) analysis of the molecular weight profile of proteins that make delta endotoxin or analysis of plasmid DNA encoding the endotoxin electrophoresis on agarose gel.

Identity of impurities, additivies and contaminants microorganismis
Contaminating microorganisms
1. Salmonella spp
2. Listeria monocytoides
3. Echerichia coli or thermotolerant (fecal) coliform
4. Staphylococcus aureus
5. Pseudomonas aeruginosa
6. Clostridium perfrigens or sulphate-reducing clostridia
7. Total number of yeasts and molds