USA EPA Plant Biostimulant Testing

Project Description

USA LABORATORY (California Lab Preferred)
MULTIPLE TESTING
EPA Environmental Microbiology Laboratory needed for USA EPA plant biostimulant testing of a liquid solution containing 99.8% water (at a 6 to 7 pH) and a mixture of living microbial species mostly including bacteria for CDFA registration.

We add to this mix of naturally harvested bacteria four more specific bacteria in high enough quantities so that we can claim those added bacteria as our Guaranteed Analysis ingredient for CDFA registration purpose. We intend to register thesolution as an Auxiliary Soil and Plant Substances (ASPS).

The added bacteria are:

 BACILLUS AMYLOLIQUEFACIENS
 BACILLUS LICHENIFORMIS
 BACILLUS PUMILUS
 BACILLUS SUBTILIS

We need to test and quantify how many CFU/ml of those bacillus bacteria remain in the final solution through plating. Note that Bacillus bacteria survive pasteurization, which may help reducing the many other bacteria present in the solution, hence simplifying the counting process.

Additionally, we would like to re-perform the following tests:

1) Heavy Metal
2) Coliform (total and fecal)
3) Salmonella
4) Listeria
5) E. coli O157:H7

Biostimulant definition (currently proposed by the USDA/EPA): A plant biostimulant is a substance(s), microorganism(s), or mixture(s) thereof, that, when applied to seeds, plants, the rhizosphere, soil or other growth media, act(s) to support a plant's natural nutrition processes independently of the plant biostimulant's nutrient content. The plant biostimulant thereby improves nutrient availability, uptake or use efficiency, tolerance to abiotic stress, and consequent growth, development, quality, or yield.