Laboratory Test Request

Pyruvate Dehydrogenase ANtigen Complex Protein Detection

Project Description

Bioanalytial Laboratory needed for western blot service quote for data and analysis on a few blood samples (2-3 samples) using the immunoprecipitation (IP-WB) / pMIT3 antigen method for detecting the four proteins (X/E3BP, E1a, E1ß, and E2/E3) of the pyruvate dehydrogenase antigen complex.  I am particularly interested in detecting E1a. 

Are you capable of performing the same or similar assays as described in the following articles by CERIBELLI, MOTEKI, and NAKAGAMI? I am interested in using a bovine-based method rather than rat-based, unless you recommend otherwise. 

1) CERIBELLI, Detection of anti-mitochondrial antibodies by immunoprecipitation in patients with systemic sclerosis, 2018,
DOI: 10.1016/j.jim.2017.10.001 
https://air.unimi.it/handle/2434/565940  

2) MOTEKI, Use of a designer triple expression hybrid clone for three different lipoyl domain for the detection of antimitochondrial autoantibodies, 1996,
DOI: 10.1002/hep.510240117 
https://pubmed.ncbi.nlm.nih.gov/8707289/

3) NAKAGAMI Anti-PDHA1 antibody is detected in a subset of patients with schizophrenia, 2020,
DOI: 10.1038/s41598-020-63776-0 
https://pubmed.ncbi.nlm.nih.gov/32404964/

Please let me know your quote and turnaround time.

Project Information

Number:22-01130

Industry:Biotechnology

This request is closed and is no longer accepting quotes. Register or Log In to receive alerts when similar requests open in the future.
Biotechnology,Blood and Blood Products,Life Sciences,Pharmaceuticals ,

Test Request: 25-01004

I need β-glucan extraction from Reishi mushrooms (Ganoderma lucidum). view


Test Request: 25-00961

MULTIPLE TESTING & SAMPLES

BUDGET APPROVED ($2,000-$5,000)

Seeking a laboratory to run a permeability ass... view


Test Request: 25-00881

I would like to know if you can evaluate the effect of a liquid ayurvedic formulation on an osteoporosis model i... view