Standards / Methodologies:
Our standard procedure to de novo sequence the antibody protein consists of the following steps.
- Digest the antibody protein into overlapping peptides with multiple enzymes.
- Analyze the digestions with LC-MS/MS using a Thermo Orbitrap mass spectrometer.
- Characterize overlapping peptides with de novo peptide sequencing.
- Automated antibody sequence assembly from characterized peptides.
- Result examination and annotation by our scientists.
