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Protagen AG

Otto-Hahn-Str. 15

Dortmund,, 0 44227 DE

Protagen AG Services:

Long lasting experience in development and performance of various ELISA tests. Unique patent protected technology for antibody validation and selection by the UNIchipĀ® protein biochips product family.

1D Gel Electrophoresis
Can be performed under reducing and non-reducing conditions. Separation length of up to 30 cm, various staining protocols can be applied.

2D Gel Electrophoresis
High resolution protein separation via 2D gels with maximal size of 40 x 30 cm. Various staining protocols can be applied.

Western Blotting
Technique can be used in combination with 1D or 2D gel electrophoresis. Antibody selection and validation via unique in-house technology by protein biochips.

State-of-the-art equipment with method specific accessories, e.g. HPAED detector for glycosylation analysis

Amino Acid Analysis
Standard amino acid analysis as well as modified time-resolved analysis for better accuracy of quantification.

Protein Quantification
Microtiter plate based quantification technique, fully validated and developed by Protagen AG, with excellent reproducibility and high robustness.

Measurements at one specific wavelength for scanning or acquisition of multi-wavelength spectra.

Mass Spectrometry (MS) via MALDI-MS/MS or LC-ESI-MS/MS
a.) Peptide Map, both Peptide Mass Fingerprint (PMF via MS) and Peptide Fragmentation Fingerprint (PFF via MS/MS) to confirm identity of proteins. Our MS delivers more information of analyzed proteins than the officially recommended test method by HPLC.

b.) N- and C-terminal sequencing for sequence determination of entire proteins from both termini. Especially for efficient sequence determination from the C-terminus, Protagen AG has developed and applies a powerful and robust mass spectrometric technique, using In-Source Decay (ISD).

c.) LC-ESI-MS/MS, especially for complex protein mixtures, hydrophobic proteins and/or in-depth protein analysis.