Laboratory Test Request Details

Life Sciences Test Request

Request: 18-01831

Status: Closed


M. incognita nematodes Inhibition Efficacy


Life Science Company needs Laboratory for standard assay evaluating the efficacy of a compound for nematode (M. incognita) inhibition.
Method example;
The effects of selected compounds on hatching were assessed with M. incognita in 96-well microtiter plates. Eggs (ca. 50 per well) were incubated at 26?Cin45mgmL-1 in ADW of Frac- tion F, Compound 1, or citric acid standard. Control eggs were incubated in ADW. Each treatment plus control was replicated 5 times in each of two experiments except for Compound 1
(limited quantity allowed use in experiment 1 only, 4 replicates). After 8 days (experiment 1) or 7 days (experiment 2), all J2 that hatched during the incubation periods were counted and scored for mobility (see below). Data were pooled within treatment across both experiments (N = 10 for all treatments except Com- pound 1, N = 4). Hatch levels were estimated by using the mean number of J2 across all control wells as a benchmark value. Per- cent hatch within each treatment well replicate was calculated as [(J2T/J2CM) × 100],where T= treatment andCM=control mean.

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