Preclinical Cancer Studies on Cell Lines


Laboratory Test Request Description

USA OR EUROPE LABORATORY (Prefer Small Laboratory)
MULTIPLE TESTS
US or Europe Preclinical Contract Research Organization CRO needed for the following cancer studies on cell lines:

STUDY 1
Type of Cancer: Pancreatic
ATCC Cell Line: PANC-1
Cell Media: 10% fetal bovine serum, 2 mM L-glutamine and 1% antibiotic-antimycotic solution (10 units/:L penicillin, 10 :g/:L streptomycin and 25 :g/mL amphotericin. Keep cells at 37°C in a humidified atmosphere of 5% CO2.

Stage 1A
Plating: Plate at a density of 1.E+04 in 24-well cell culture plates. Allow to adhere for 24 h.
Incubation: Incubated with vehicle (media) or 1mM 5-FU for 72 h. All experiments to be conducted in triplicate.
Post Incubation: After incubation, detach cells with trypsin and count cell numbers. IMMEDIATELY proceed to Stage 1B.

Stage 1B
Plating: Plate surviving cells at a density of 5.E+03 in 24-well cell culture plates and allow to adhere for 24 h
Incubation: Incubate 14 days with the following:
1) Vehicle (media)
2) 5-FU at 1mM
3) Gefitinib at 1µM
4) Compound A at 1mg/ml
5) Combined #3 and #4
6) Compound B at 2mg/ml
7) Combined #3 and #6
Incubation Notes: Compounds A & B to be furnished by sponsor. All treatments to be conducted in triplicate. Media to be refreshed with the respective treatments every 72 hours until the time of termination (14 days).
Post Incubation: Cells to be trypsinized and counted. Report mean cell numbers and S.E.

---------------------------------------------

STUDY 1
Type of Cancer: Non-small Cell Lung
ATCC Cell Line: A549
Cell Media: 10% fetal bovine serum, 2 mM L-glutamine and 1% antibiotic-antimycotic solution (10 units/:L penicillin, 10 :g/:L streptomycin and 25 :g/mL amphotericin. Keep cells at 37°C in a humidified atmosphere of 5% CO2.

Stage 1A
Plating: Plate at a density of 1.E+04 in 24-well cell culture plates. Allow to adhere for 24 h.
Incubation: Incubated with vehicle (media) or 6.6 µM cisplatin for 72 h. All experiments to be conducted in triplicate.
Post Incubation: After incubation, detach cells with trypsin and count cell numbers. IMMEDIATELY proceed to Stage 1B.

Stage 1B
Plating: Plate surviving cells at a density of 5.E+03 in 24-well cell culture plates and allow to adhere for 24 h
Incubation: Incubate 14 days with the following:
1) Vehicle (media)
2) 5-FU at 1mM
3) Gefitinib at 1µM
4) Compound A at 1mg/ml
5) Combined #3 and #4
6) Compound B at 2mg/ml
7) Combined #3 and #6
Incubation Notes: Compounds A & B to be furnished by sponsor. All treatments to be conducted in triplicate. Media to be refreshed with the respective treatments every 72 hours until the time of termination (14 days).
Post Incubation: Cells to be trypsinized and counted. Report mean cell numbers and S.E.


INFORMATION

Number: 21-03018

Industry: Pharmaceuticals



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