FDA GMP Analytical Chemistry Laboratory needed for FDA Compliance testing of Yellow #5 and its compounds in fried potato chips:
Sum of volatile matter at 135 °C (275 °F) and chlorides and sulfates (calculated as sodium salts), not more than 13 percent.
· Water-insoluble matter, not more than 0.2 percent.
· 4,4´-[4,5-Dihydro-5-oxo-4-[(4-sulfophenyl)hydrazono]-1H-pyrazol-1,3-diyl]bis[benzenesulfonic acid], trisodium salt, not more than 1 percent.
· 4-[(4´,5-Disulfo[1,1´-biphenyl]-2-yl)hydrazono]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylic acid, tetrasodium salt, not more than 1 percent.
· Ethyl or methyl 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)hydrazono]-1H-pyrazole-3-carboxylate, disodium salt, not more than 1 percent.
· Sum of 4,5-dihydro-5-oxo-1-phenyl-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid, disodium salt, and 4,5-dihydro-5-oxo-4-(phenylazo)-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylic acid, disodium salt, not more than 0.5 percent.
· 4-Aminobenzenesulfonic acid, sodium salt, not more than 0.2 percent.
· 4,5-Dihydro-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylic acid, disodium salt, not more than 0.2 percent.
· Ethyl or methyl 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylate, sodium salt, not more than 0.1 percent.
· 4,4´-(1-Triazene-1,3-diyl)bis[benzenesulfonic acid], disodium salt, not more than 0.05 percent.
· 4-Aminoazobenzene, not more than 75 parts per billion.
· 4-Aminobiphenyl, not more than 5 parts per billion.
· Aniline, not more than 100 parts per billion.
· Azobenzene, not more than 40 parts per billion.
· Benzidine, not more than 1 part per billion.
· 1,3-Diphenyltriazene, not more than 40 parts per billion.
· Lead (as Pb), not more than 10 parts per million.
· Arsenic (as As), not more than 3 parts per million.
· Mercury (as Hg), not more than 1 part per million.
· Total color, not less than 87 percent.
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