Laboratory Test Request

Zeaxanthin and Meso-Zeaxanthin Assay Confirmation Testing

Project Description

Dietary Supplement Laboratory needed to confirm assay percentage of Zeaxanthin and meso-zeaxanthin (meso-zeaxanthin as a portion of zeaxanthin in a zeaxanthin raw material)? I found this in an NIH study, and wonder if you do the same “two successive HPLC separations”? I ask this because we have a label that calls out not only Lutein, but also Zeaxanthin AND Meso-zeaxanthin with meso-Zeaxanthin being 10 of the 12 mg of the zeaxanthin (see attached).

Identification and quantification of the macular carotenoids (from https://pmc.ncbi.nlm.nih.gov/articles/PMC3740325/)
“To quantify MZ, L, and Z, current methodology requires two successive HPLC separations (assays). The first assay (reverse phase chromatography) separates L from a total Z fraction, which contains Z and MZ. This total Z fraction is typically manually collected, and then analysed using the second assay (normal phase chromatography). This second assay is required to separate Z and MZ, so that quantification of these individual carotenoids can be achieved. This general method of carotenoid analysis has been adapted and modified by several laboratories for the purpose of MZ quantification.21, 27, 28, 29, 30 Modifications are typically made to the first assay, where a variety of reverse phase columns and solvent compositions can produce acceptable L and total Z separations.31 However, the normal phase column (Daicel ChiralPak AD (Daicel Chiral Technologies Europe, Illkirch – Cedex, France); 250 × 4.6 mm2) used in the second assay has remained constant and universal among studies reporting on MZ.”

Project Information

Number:24-02540

Industry:Dietary Supplements and Nutraceuticals

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