Pharmaceutical Preclinical Laboratory needed for four different in-vitro and in-vivo research experiment research studies:
1) In vivo-Balb/c mice CLP model- First experiment of about 100 animals, readout will be survival. Possibly additional experiments in this model where additional outcomes will be desired: e.g. cytokines, T cell distribution, body temperature, apoptosis.
2) Kinetics-Determine the residence time of our drug on its target (transfected cell and T cells)
3) In vitro- measured the time required for our drug to exert its effect as monitored using cytokine response in PBMCs
4) Microscopy/FACS- Determine the ability of our drug to internalize into cells (by using a fluorescent labeled drug and FACS/microscopy techniques)
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