Laboratory Test Request Description

India Pharmaceutical Science Researcher needs Pharmacology Contract Research Organization for Pharmacology Study Experiments:

AIM AND OBJECTIVES OF THE SCIENTIFIC REASERCH WORK
The study has been designed to serve the following objectives:
1. To explore the critical role of selected antioxidants, minerals and vitamins on gastro protection by using various antiulcer screening models and biochemical parameters in rats.
2. To compare the effect of selected antioxidants, minerals and vitamins in GI disorders along with other reference gold standard drugs.
3. To recommend a safe therapy in managing gastric disorders based on preclinical studies.

METHODOLOGY OF THE REASERCH WORK
? Screening models:
1. Ethanol- Induced Ulcer Model in rats.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
? Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
2. Pylorus Ligation Induced Ulcer Model in rats.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
? Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
3. Cold Restraint stress Induced Ulcer (CRU) Model in rats.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
? Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
4. A small animal model of human Helicobacter pylori infection.
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes
5. Indomethacin induced ulcer model
Determination of Acid Secretary Parameters in ethanol-administered rats.
? Determination of Ulcer Score.
? pH of the gastric juice.
? Volume of the gastric juice.
? Free and total acidity in gastric juice.
? Histopathology of the isolated stomach.
Biochemical Investigations.
? Determination of pepsin activity.
? Determination of mucin content10.
? Determination of myeloperoxidase activity.
Estimation of lipid peroxides, reduced glutathione and antioxidant enzymes


following 8 Groups of animals (n = 6) will be used in study


INFORMATION

Number: 16-00183

Industry:



Interested in learning more about this
Laboratory Test Request?


Unlock more information about this
Laboratory Test Request


Pharmaceuticals and Biopharmaceuticals

Need Testing or Scientific Research?

Save time and money searching. Simply, submit a request to find the laboratories or lab suppliers that best meet your needs.

Submit Laboratory Request

Test Request: 20-00850

Asia Contract Laboratory needed for air purifier testing to one of the following 3 Test Standards: 1. JEM Japan 2. AH... view


Test Request: 20-00661

Organization looking to purchase the following for their laboratory ;01 X VIRAL LOAD MACHINE C/W MONITOR/PRINTER/REAGENT... view


Product Request: 20-00622

Pharmaceutical Drug Discovery Company needs supplier of freshly isolated human platelets. I can provide further details ... view