Laboratory Test Request Details

Test Request

Request: 14-01361

Status: Closed


Canada ISO bioanalytical laboratory needed forbioanalytical research study for Evaluation of the effect of crude oil contaminants as in-vitro endocrine disruptor and cell proliferation
This research will evaluates the potential endocrine disruptor activity of substances by measuring whether and to what extent a substance induces cell proliferation via estrogen receptor (ER), androgen receptor (AR) (PR) dependent pathways. This research is aimed at screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human androgen receptor and estrogen (ER?) estrogen (ER?).
We shall assay for the following:
1. Agonist dose-response of the AR Reporter Assay
2. Antagonist dose-response of the AR reporter Assay
3. Human ER? and ER? Agonist assays
4. Human ER? antagonist
5. Human ER? antagonist Assay
the statistical analysis shall be carried out as outline below.
Average relative light units (RLU) and corresponding standard deviation (SD) values will be determined for each model concentration (n ? 6). Signal-to-background (S/B) and Z? values will be calculated using Hill?s equation as described by Zhang, et al. (1999a). Non-linear regression and regression analysis, inhibitory concentration (IC50) as well as (Ki) and effective concentration (EC50) analysis will be performed using GraphPad Prism version 6 software. Low percentage coefficient of variation (% CV), high S/B and Z scores will confirm the robust performance of this Assay.

Interested in this Request?

Members of our Laboratory Outsourcing Network can use our online platform to communicate with the Lab Requestor, submit responses with quotes and have access to more information such as budget, location required, etc.

Create FREE Account

Oil, Petroleum and Gasoline

Need Testing or Scientific Research?

Save time and money searching. Simply, submit a request to find the laboratories or lab suppliers that best meet your needs.

Submit Laboratory Request

Business Request: 19-01020

LEGAL /LONG-TERM TESTING Drug Screening Company looking for USA Clinical Laborator ywith MRO for hair testing and Peth t... view

Test Request: 19-00955

Contract Sterilizer needed for contract packaging and gamma sterilization for a small batch (around 200 units) of bone v... view

Product Request: 19-00949

University Scientific Researcher needs Laboratory Supplier of defibrinated rabbit blood. view