Contract Laboratory has received the following incoming Laboratory Test Requests which need to be fulfilled! These Laboratory Test Requests are received from a variety of sources ranging from start-up companies to large, multinational Fortune 500 corporations, international organizations, well-respected universities and government agencies that are actively seeking Independent, Third-Party Molecular Biology Laboratories to perform their Molecular Biology Testing, Analysis, Assays, Product Development, Scientific Research Experiments, Inspections, Certifications, and Engineering Projects. If you are a Molecular Biology laboratory that would like more information on these Molecular Biology projects, please visit Register Laboratories or call toll free 1-855-377-6821.
Molecular Biology / Bioanalytical Laboratory needed for up to three university-based experiments involving human cell lines:
Experiment #1: Test up to 5 human cell lines (i.e. liver, kidney, etc...). Formalinize these cell lines in order to have a bank ready to be used. Then culture peripheral blood stem cells,apply the different formalin treated cells into the growth media, and determine what occurs. I am looking to determine if differentiation is stimulated toward the cell lines that were added. Is there inhibition or is there no reaction?
Experiment #2: Culture peripheral stem cells, add various growth factors (ex. neupogen), and determine if cell expansion is stimulated
Experiment #3: Growth of autologous stem cells on an autologous feeder layer (cells and feeder layer from the same patient). Looking to find what numbers
LONG-TERM TESTING Bioanalytical laboratory needed for molecular biology experiments that we design the protocols for including Liquid Handling - Single Channel Liquid Handling - Multi Channel (Stamping) Miniprep Centrifugation Gel Electrophoresis Incubation / shaking (37C) Measurement (e.g. volume, dna concentration) Spectrophotometry - Absorbance, Fluorescence, Luminescence Spreading Bacteria & Colony Picking Thermocycling Storage (Ambient, 4C, -20C, -80C, 37C)
Below is a list of sample experiments that we have sent to our current provider. This is not a complete list. We would design these to work well with the standard containers that your lab is used to working with but the protocols we provide will be this detailed. PCR, DNA ligation, bacteria counting, bacteria freezing, gel purify a pcr product, miniprep, sanger sequ
Bioanalytical Laboratory needed for Product development work for molecular biology (PCR, library prep, DNA sequencing on Illumina platform). We need help with verification testing using a molecular laboratory. Must follow protocols and generate data for reports to be submitted to FDA as part of design control.
University Researcher needs Africa botany laboratory for Plant molecular biology studies on the microbiology and interactions plant-microorganisms. Study includes Plant quality analysis, Plant physiology surveys, Plant biochemistry, Plant nutrition, Plant genetics, Plant and soil, etc.
US ISO ad FDA GMP Molecular Biology Laboratory needed for in-vitro diagnostic IVD product development feasibility and development work. Expect project to take less than 6 months. Tight timeline.
Need to work direction of Group Lead at our company.
LARGE AGRICULTURE CHEMICAL MANUFACTURER NEEDS MOLECULAR BIOLOGY LABORATORY FOR MOLECULAR BIOLOGY TESTING
India Pharmaceutical Molecular Biology Laboratory needed for molecular biology testing
France ISO and CLIA bioanalytical laboratory needed for CLIA molecular biology assay (IVD / LDT) development: design optimization, analytical performance evaluation, pre-validation on archived clinical samples to ISO 15189 and CLIA
Israel medical diagnostics manufacturer needs . East Coast CLIA laboratory molecular biology (QRT PCR)
USA FDA GMP Molecular biology laboratory needed for hormone radioimmunoassay: 10-15 samples of mice blood for radioimmunoassay of hormone ghrelin.
Argentina FDA GLP Molecular Biology Laboratory needed for GMO testing (Genetically Modified) presence testing in corn
USA Molecular Biology Laboratory needed for complete Proteomic Analysis of different cell types and tissues
USA/Canada or Europe Molecular Biology Laboratory needed for Southern Blottting Testing on plant samples using supplied protocol.
USA Molecular Biology Laboratory needed for protein detection testing for leaf samples through Western blot technique. We shall provide the primary antibodies. Number of samples will be 20.
USA Molecular Biology Laboratory needed for Southern Blot analysis, probe will be provided. Would also like a price for the restriction digestion of the genomic DNA prior to the southern.
Asia (preferably Malaysia, Singapore) Molecular Biology Laboratory needed for southern blot, P32, 300 samples immediately, ~100 per month for the next 12 months
ISA Molecular Biology Laboratory needed for genetic transformation testing of silkworm for producing recombinant protein of spider.
WestCoast USA Molecular Biology Laboratory needed to have a frozen sample of bone marrow derived cells thawed and analyzed to determine viability plus the type and numbers of stem & progenitor cells present (Mesenchymals, CD34++, etc)
Molecular Biology Laboratory needed for Yeast protein expression, mammalian cell transfection
Soth Africa Molecular Biology Laboratory needed for testing three cell lines namely, the murine derived multipotent stem cell line- C17.2, BV 2 microglia and PC-12 cells
UK Molecular Biology needed for characterisation of novel nucleoside/nucleotide base units present in KOH-neutralised perchloric acid cell extracts. The techniques required would be LC-MS (and possibly LC-NMR, LC-MS/NMR, as NMR profiles would already be available for identifying chromatographic peaks). We would need MS fragmentation patterns so as to identify components using libraries, etc.
US GLP Molecular Biology Laboratory needed for immunochemistry testing. SDS-PAGE, Western Blot and titer out antigen.
Preclinical CRO, molecular biology laboratory needed for ligand binding assay novel AT1 receptor antagonists, binding affinity and specificity for the AT1 receptor. As these drugs will be tested in rats, selectivity for AT1A versus AT1B and AT2 receptors needs to be elucidated.
US or Europe Non-GLP Molecular Biology Laboratory required for overlapping large insert BAC recombineering into larger BACs, generation of inpu custom BAC library if possible in same lab but not required
Molecular Biology Laboratory needed for RAPD analysis of two ecotypes of duck using blood and estimating genetic distance btw the ecotypes bases on band sharing using random primers
Northeast Clinical Molecular Biology Laboratory needed for transcription factor, cell testing from 30-40 human blood samples, 5-10 transcription factors using TransAm Transcription Factor
New York Molecular Biology/Clinical Laboratory needed for PRC, PCR-TR, microarray, and electrophoresis
Molecular Biology Contract Research Organization needed to study the effect on gene expression of a Herpes Simplex Virus (HSV) structural protein. The experimental design is quite straightforward where we want to use siRNA to silence the expression of a specific HSV protein and measure the overall effect on HSV infection/gene expression. While we have done similar experiments inhouse we are moving towards moving these basic assays to an outsourced model so that we can achieve maximal momentum by orienting our resources to other projects.
Molecular biology laboratory needed for testing of cell lines. Expression of a mammalian transmembrane receptor for protein isolation and subsequent binding assays.
Bioanalytical molecular biology laboratory needed for testing for permeability (unidirectional caco 2 cell line)of a few drug samples
Molecular Biology laboratory needed for partial digest to remove a synthetic gene in a vector and clone into another vector cut with compatible restriction sites. We will provide both vectors. One vector is 16.5kb and the other is 1.6 kb, no unusual sequences. No published complete sequence for the vector.
Molecular biology laboratory needed for testing for presence/absence of nucleases (RNASE and DNASe) and endotoxins
BSL-3 microbiology and molecular biology laboratory needed for exposure of blood mononuclear cells to organizms and then extracting the RNA. The project would involve exposure of peripheral blood mononuclear cells (PBMC) to either Francisella tularensis or Yersinia pestis, incubation and isolation of RNA from these cells. In order to accomplish this we would need to have the bacteria (F. tularensis and Y. pestis) grown in liquid culture, harvested and resuspended in appropriate media. The bacteria at appropriate concentrations would then be incubated with PBMCs that would be provided. After co-incubation, PBMCs will need to be separated from the bacteria and RNA extracted from the PBMCs. Since the bacteria involved are category A pathogens, BSL-3 level containment is required for all procedures, until isolation of the PBMC RNA.