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Contract Laboratory has received the following Molecular Biology Laboratory Test Requests which need to be fulfilled! These Laboratory Test Requests are received from a variety of sources ranging from start-up companies to large, multinational Fortune 500 corporations, international organizations, well-respected universities and government agencies that are actively seeking Independent, Third-Party Molecular Biology Laboratories to perform their Molecular Biology Testing, Analysis, Assays, Product Development, Scientific Research Experiments, Inspections, Certifications, and Engineering Projects. If you are a Molecular Biology laboratory that would like more information on these Molecular Biology projects, please visit our registration page or call toll us free 1-855-377-6821.
UK Molecular Biology needed for characterisation of novel nucleoside/nucleotide base units present in KOH-neutralised perchloric acid cell extracts. The techniques required would be LC-MS (and possibly LC-NMR, LC-MS/NMR, as NMR profiles would already be available for identifying chromatographic peaks). We would need MS fragmentation patterns so as to identify components using libraries, etc.
Preclinical CRO, molecular biology laboratory needed for ligand binding assay novel AT1 receptor antagonists, binding affinity and specificity for the AT1 receptor. As these drugs will be tested in rats, selectivity for AT1A versus AT1B and AT2 receptors needs to be elucidated.
Molecular Biology Contract Research Organization needed to study the effect on gene expression of a Herpes Simplex Virus (HSV) structural protein. The experimental design is quite straightforward where we want to use siRNA to silence the expression of a specific HSV protein and measure the overall effect on HSV infection/gene expression. While we have done similar experiments inhouse we are moving towards moving these basic assays to an outsourced model so that we can achieve maximal momentum by orienting our resources to other projects.
Molecular Biology laboratory needed for partial digest to remove a synthetic gene in a vector and clone into another vector cut with compatible restriction sites. We will provide both vectors. One vector is 16.5kb and the other is 1.6 kb, no unusual sequences. No published complete sequence for the vector.
BSL-3 microbiology and molecular biology laboratory needed for exposure of blood mononuclear cells to organizms and then extracting the RNA. The project would involve exposure of peripheral blood mononuclear cells (PBMC) to either Francisella tularensis or Yersinia pestis, incubation and isolation of RNA from these cells. In order to accomplish this we would need to have the bacteria (F. tularensis and Y. pestis) grown in liquid culture, harvested and resuspended in appropriate media. The bacteria at appropriate concentrations would then be incubated with PBMCs that would be provided. After co-incubation, PBMCs will need to be separated from the bacteria and RNA extracted from the PBMCs. Since the bacteria involved are category A pathogens, BSL-3 level containment is required for all procedures, until isolation of the PBMC RNA.
Pharmaceutical Laboratory needed for Residual Moisture Content analysis (e.g. Karl-Fisher analysis ) on lyophilized cake. All excipients in the formulation safely handled under BL 1 conditions. Fast Turn Around Times a must. Laboratory should be in the contiguous United States.
Bioanalytical Laboratory needed for Vitamin detection on Fetal Bovine Serum. Following is a list of vitamins and detectable units:
Vitamin B-12 pg/mL
Folic Acid ng/mL
Vitamin B-6 ng/mL
Thiamin B-1 ng/mL
Vitamin H Biotin pg/mL
Riboflavin B2 ng/mL
Vitamin A Retinol ng/mL
Vitamin C ng/mL
Microbiology Laboratory needed for microbial analysis SB 3112. The test material consisted of only the bacterial spores at a count of 55 x 107 cfu/ml. The tests were run in compliance with the following protocols: 1 USEPA FIFRA;GLP Standards 40 CFR 160 2 USEPA TSCA 40 CFR 792 3 Org. Economic Coop. and Dev. Principles of GLP, Annex 2, C(97)186 4 Japan Ministry of Ag, Forestry & Fisheries, Notification No. 59 Noshan 3850, Director-General of Agricultural Prod. Bureau, 10 Aug 84
Preclinical toxicology laboratory needed for Cytotoxicity studies: MEM Elution Sample Amount: 120 cm2 or 4 grams Irritation: Intracutaneous Reactivity (ISO) (w/2 extracts) Sample Amount: 240 cm2 or 8 grams Sensitization: Magnusson-Kligman (w/2 extracts) Sample Amount 600 cm2 or 32 grams (6 samples minimum) Sensitization: Local Lymph Node Assay: Sample Amount 400 cm2 or 20 grams