Contract Laboratory has received the following Molecular Biology Laboratory Test Requests which need to be fulfilled! These Laboratory Test Requests are received from a variety of sources ranging from start-up companies to large, multinational Fortune 500 corporations, international organizations, well-respected universities and government agencies that are actively seeking Independent, Third-Party Molecular Biology Laboratories to perform their Molecular Biology Testing, Analysis, Assays, Product Development, Scientific Research Experiments, Inspections, Certifications, and Engineering Projects. If you are a Molecular Biology laboratory that would like more information on these Molecular Biology projects, please visit our registration page or call toll us free 1-855-377-6821.
Molecular Biology / Bioanalytical Laboratory needed for up to three university-based experiments involving human cell lines:
Experiment #1: Test up to 5 human cell lines (i.e. liver, kidney, etc...). Formalinize these cell lines in order to have a bank ready to be used. Then culture peripheral blood stem cells,apply the different formalin treated cells into the growth media, and determine what occurs. I am looking to determine if differentiation is stimulated toward the cell lines that were added. Is there inhibition or is there no reaction?
Experiment #2: Culture peripheral stem cells, add various growth factors (ex. neupogen), and determine if cell expansion is stimulated
Experiment #3: Growth of autologous stem cells on an autologous feeder layer (cells and feeder layer from the same patient). Looking to find what numbers of cells are achieved in a set time frame.
LONG-TERM TESTING Bioanalytical laboratory needed for molecular biology experiments that we design the protocols for including Liquid Handling - Single Channel
Liquid Handling - Multi Channel (Stamping)
Incubation / shaking (37C)
Measurement (e.g. volume, dna concentration)
Spectrophotometry - Absorbance, Fluorescence, Luminescence
Spreading Bacteria & Colony Picking
Storage (Ambient, 4C, -20C, -80C, 37C)
Below is a list of sample experiments that we have sent to our current provider. This is not a complete list. We would design these to work well with the standard containers that your lab is used to working with but the protocols we provide will be this detailed.
PCR, DNA ligation, bacteria counting, bacteria freezing, gel purify a pcr product, miniprep, sanger sequence, transform, spread, pick, gibson assembly followed by transform/spread/pick colonies, make bacteria chemically competent
Bioanalytical Laboratory needed for Product development work for molecular biology (PCR, library prep, DNA sequencing on Illumina platform). We need help with verification testing using a molecular laboratory. Must follow protocols and generate data for reports to be submitted to FDA as part of design control.
University Researcher needs Africa botany laboratory for Plant molecular biology studies on the microbiology and interactions plant-microorganisms. Study includes Plant quality analysis, Plant physiology surveys, Plant biochemistry, Plant nutrition, Plant genetics, Plant and soil, etc.
UK Molecular Biology needed for characterisation of novel nucleoside/nucleotide base units present in KOH-neutralised perchloric acid cell extracts. The techniques required would be LC-MS (and possibly LC-NMR, LC-MS/NMR, as NMR profiles would already be available for identifying chromatographic peaks). We would need MS fragmentation patterns so as to identify components using libraries, etc.
Preclinical CRO, molecular biology laboratory needed for ligand binding assay novel AT1 receptor antagonists, binding affinity and specificity for the AT1 receptor. As these drugs will be tested in rats, selectivity for AT1A versus AT1B and AT2 receptors needs to be elucidated.
Molecular Biology Contract Research Organization needed to study the effect on gene expression of a Herpes Simplex Virus (HSV) structural protein. The experimental design is quite straightforward where we want to use siRNA to silence the expression of a specific HSV protein and measure the overall effect on HSV infection/gene expression. While we have done similar experiments inhouse we are moving towards moving these basic assays to an outsourced model so that we can achieve maximal momentum by orienting our resources to other projects.
Molecular Biology laboratory needed for partial digest to remove a synthetic gene in a vector and clone into another vector cut with compatible restriction sites. We will provide both vectors. One vector is 16.5kb and the other is 1.6 kb, no unusual sequences. No published complete sequence for the vector.
BSL-3 microbiology and molecular biology laboratory needed for exposure of blood mononuclear cells to organizms and then extracting the RNA. The project would involve exposure of peripheral blood mononuclear cells (PBMC) to either Francisella tularensis or Yersinia pestis, incubation and isolation of RNA from these cells. In order to accomplish this we would need to have the bacteria (F. tularensis and Y. pestis) grown in liquid culture, harvested and resuspended in appropriate media. The bacteria at appropriate concentrations would then be incubated with PBMCs that would be provided. After co-incubation, PBMCs will need to be separated from the bacteria and RNA extracted from the PBMCs. Since the bacteria involved are category A pathogens, BSL-3 level containment is required for all procedures, until isolation of the PBMC RNA.