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Neuroscience Research Laboratory at Methodist Research Institute
1800 N Capitol Ave,
Indianapolis, IN 46202 US
We have state-of-the-art facility to conduct bioanalytical, preclinical, drug testing, research and development.
Tissue culture facility to culture, grow and maintain immune cells, tumor cells and stem cells;
Cell biology facility to measure cell proliferation by thymidine uptake or Wst-1 assay, cell cycle progression by flow cytometry, apoptotic cell death by Tunel assay.
Molecular biology facility to detect gene expression, genotyping, gene transfection and analysis by PCR and real-time PCR, agarose gel electrophoresis.
Biochemical and immunological techniques including, ELISA, Western blot, immunohistochemistry, intracellular cytokine and surface protein detection.
Signal transduction techniques including protein phosphorylation and DNA binding assay.
Animal facility to perform preclinical drug testing in EAE model of multiple sclerosis, inflammation models and xenograft models of cancer and cancer stem cells.