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Neuroscience Research Laboratory at Methodist Research Institute

1800 N Capitol Ave,

Indianapolis, IN 46202 US

The Neuroscience Research Laboratory investigates mechanisms in the regulation of growth, immune and inflammatory responses relevant to multiple sclerosis (MS), cancer, stem cell biology, inflammation and autoimmune diseases. We use state-of-the-art cell biology, immunology and biochemistry molecular biology techniques to discover novel inflammatory signatures, biomarkers and drug targets for human diseases.

Preclinical testing: We develop stem cell transplant therapies for neurodegenerative diseases. We study self-renewal, growth and angiogenesis of cancer stem cells. We identify biomarkers and develop novel therapies to target cancer and cancer stem cells and for immune, inflammatory and autoimmune diseases. We use relevant animal models of multiple sclerosis, cancer and inflammation for preclinical testing.

Bioanalytical testing: We measure cytokine and other biomolecules, cell surface markers, gene expression, genotyping, proliferation, cell cycling, cell death, apoptosis, necrosis, angiogenesis, cell culture and maintenance, flow cytometry.

Technical Information:

We have state-of-the-art facility to conduct bioanalytical, preclinical, drug testing, research and development.

Tissue culture facility to culture, grow and maintain immune cells, tumor cells and stem cells;

Cell biology facility to measure cell proliferation by thymidine uptake or Wst-1 assay, cell cycle progression by flow cytometry, apoptotic cell death by Tunel assay.

Molecular biology facility to detect gene expression, genotyping, gene transfection and analysis by PCR and real-time PCR, agarose gel electrophoresis.

Biochemical and immunological techniques including, ELISA, Western blot, immunohistochemistry, intracellular cytokine and surface protein detection.

Signal transduction techniques including protein phosphorylation and DNA binding assay.

Animal facility to perform preclinical drug testing in EAE model of multiple sclerosis, inflammation models and xenograft models of cancer and cancer stem cells.

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